** Introduction : A medical microbiology lab performs testing on animal samples calm from altered anatomy sites. The tests are acclimated to ascertain and analyze any microorganisms able of causing disease. Alive of alien bacillus is important on how this bacillus works and how it is structured, agency alive how it can affect humans. The purpose of this abstraction was to analyze an alien bacillus by applying all methods that were ahead conducted and abstruse in the medical microbiology chic class. **Materials : 1) Claret agar bowl . 2) Mannitol Alkali agar (MSA) plate. ) DNase agar bowl . 4) Novobiocin disc . 5) Inoculating loop. 6) blaze ( Bunsen burner) . 7) 1N hydrochloric acerbic (HCl) . 8) Two slides . 9) Plasma tube. 10) 3% Hydrogen Achromatize (H2O2) . 11) One alien bowl . 12) Crystal violet. 13) Gram’s iodine . 14) Safranin. 15) Alcohol . ** Methods : An alien labeled with cardinal 8 was accustomed out by the lab instructor. The ambition at this point was to actuate alien gram absolute vacteria. The procedures performed consisted of antiseptic address in accession to actuality followed as declared in the referenced advance chic chiral by Matar (1) , unless contrarily noted.
Not all of the tests were performed on every culture. However, there are as some of the tests were acclimated alone for gram (+) others were alike added specific and acclimated alone for cocci bacilli . The aboriginal action accept been done was to beam and almanac the analysis of the alien sample. However, Gram stain should be done to be abiding that alien sample were gram absolute and to analyze beef morphology. After that biochemical tests were called for alien identification . aboriginal of all was done the catalase analysis to differentiate amid the two types of cocci bacilli ( Staphylococcus and Streptococcus ) .
Since alien 8 was bent to be Staphylococcus coagulase analysis in accession to the afterward tests were performed on this alien : 1) Assembly of DNase on DNase agar. 2) Claret agar with novobiocin (NB) analysis . 3) Mannitol beverage on Mannitol Alkali agar (MSA) . **Results : Colonies analysis on bowl was accustomed were as follows : circular, raised, smooth, opaque, white-yellow pigmens. After alive that it was Gram absolute cocci , a catalase analysis and coagulase analysis was done , in accession to altered plates evolution ( Claret agar , DNase and MSA plates ) .
The afterward table lists all of the tests were done : Test| Purpose| Reagents| Observations| Results| Gram stain| To actuate The gram rxn ofBacterium. | Crystal violet,Iodine, Alcohol&Safranin. | Purple cocci| Gram absolute Cocci . | Catalase test| To actuate ifBact. Posses catalase enzyme. | 3% H2O2| Oxygen bubblesWere observed. | Absolute catalaseTest. | Coagulase test| To ascertain thePresence of “Clumping factor”. | Plasma. | No array was Formed. | Negative coagul-ase analysis . | DNase bowl Test| To actuate ifBact. producesDNase enzyme. | 1N HCl . Cloudy breadth (notClear one ). | Negative . | Hemolysis test(blood agar). | To actuate ifBacteria do Hemolysis. | None . | No arresting Changes wereAround colonies. | Gamma hemolysis| Mannitol Fermentation. | To determineThe adeptness of Bacillus to agitate mannitol. | None . | Color changeFrom blush to Chicken . | Absolute mannitolFermenter. | Novobiocin Test| To ascertain Sensitivity or Resistance of Bact to NB Antibiotic. | NB antibacterial . | No breadth ofInhibition aroundDisc. | Aggressive bact. | Flowchart Alien 8 Gram stain Gram absolute cocci Catalse test(positive)
Positive Negative Staphylococcus aureus. Streptococcus pneumonia Staphylococcus epidermidis. Viridans Streptococci Staphylococcus saprophyticus S. pyogens S. agalactiae Coagulase and Dnase analysis (Negative) Enterococcus sp. Absolute Negative Staphylococcus aureus. Staphylococcus epidermidis. Staphylococcus saprophyticus Novobiocin test(Resistance)
Sensitive Resistance Staphylococcus epidermidis. Staphylococcus saprophyticus MSA bowl (Positive) Negative Absolute Staphylococcus epidermidis. Staphylococcus saprophyticus Staphylococcus aureus. Claret agar plate(hemolytic test) (no hemolysis) Staphylococcus saprophyticus Alien 8- S. saprophyticus **Discussion /Conclusion : It was assured that Alien 8 was S. saprophyticus . After applying Gram stain the gram absolute bacilli was cocci in appearance back beheld with a ablaze microscope so a catalase analysis was performed.
The bacilli was able to breach bottomward hydrogen achromatize aloft its accession into baptize and aerial oxygen which created bubbles and adumbrated a absolute result. A sample was again inoculated on a mannitol alkali agar plate. After evolution advance was present and the red media had angry chicken about the advance as a aftereffect of aerial levels of acerbic production. The abstracts suggests that the gram absolute bacilli was Staphylococci saprophyticus because it was gram positive, was catalase absolute with the assembly of O, and was aggressive to novobiocin disc.
Staphylococcus saprophyticus is a ache of Staphylococcus bacteria. Approximately 25 percent of individuals backpack this bacilli in the anal area, genitals, adenoids and mouth. People who airing barefoot are decumbent to access the bacilli from the floor. Staphylococcus may account an infection back the bacilli access a cut in any breadth of the body. These staph infections can ambit from boils to cannibal infections. The best accepted staph infection is Staphylococcus saprophyticus which frequently occurs in women.
This staph is one of two bacilli which can access the urinary tract. Approximately 20 percent of women who ache from a urinary amplitude infection (UTI) will accept addition infection. **References : 1) Matar, Suzan. Medical microbiology Chic Manual. Jordan: University of Jordan publishing. 2) http://www. studymode. com/subjects/unknown-lab-report-on-gram-positive-bacteria-page1. html . 3) http://en. wikipedia. org/wiki/Gram-positive_bacteria . 4) http://www. ehow. com/about_5453276_staphylococcus-saprophyticus-infection. html
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