Growing Fungi and Bacteria of Plants
Introduction There are both baneful and non-virulent bacilli and fungi that abound on plants. It is difficult to analyze amid the two after able analysis and analysis of the afflicted bulb to apperceive whether the bacilli or the bane in catechism is the baneful or non-virulent one. Therefore authentic cultures charge to be abandoned to apperceive with complete authoritativeness which is the adroit bacilli or fungus. Potato dextrose agar is a acceptable comestible agar for mycelia to advance on which is present in best fungal moulds. Accepted comestible agar is a accepted account acclimated for non-fastidious microorganisms. 2 Aim The aim is to abstract fungi and bacilli colonies from afflicted and advantageous leaves. Materials and Methods Materials acclimated for the agreement was two of each: accepted comestible agar bowl and potato dextrose agar plate. To abolish any epiphytic or saprophytic bacilli from the bulb apparent the blade is apparently antibacterial with 100% ethanol. A scalpel to cut the leaf
Sterile baptize for macerating the leaves. To abstract the fungi: Cut 5 pieces of blade from the afflicted blade about the edges of the afflicted breadth so it contains both advantageous and afflicted parts, abode it on the one potato dextrose agar plate. Then cut 5 pieces from the advantageous blade and abode them on the additional dextrose agar plates. To abstract bacteria: Macerate both the advantageous and afflicted leaves alone in the antiseptic baptize and band it assimilate the two abstracted accepted comestible agar plate.
Incubate all four plates at 25? C for up to 7 days. Discussion There is advance on both the advantageous and afflicted agar plates for both bacilli and fungi. For the advantageous fungal bowl there is a distinct blazon of advance which suggests that it is a accustomed non-pathogenic bane that grows on plants. On the afflicted plants there are 5 altered colonies of bane from the 5 altered pieces of leaves. The one breadth has a bright area area the bane has inhibited the advance of the alternative colonies.
On the advantageous bacterial bowl there is some bacterial advance which can additionally advance non-pathogenic bacilli that grows on plants. On the afflicted bulb bacterial bowl there is some bacterial advance but not a lot and still fungal growth. Conclusion The abridgement of bacterial colonies on the bacterial bowl and the fungal advance on the aforementioned bowl suggests that the ache on the bulb is acquired by a fungal antibody and not a bacterial one. There are fungi and bacilli that abound on plants that are not adverse appear the plant. ?
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