Effect of Enzyme Catalese on Hydrogen Peroxide

Aim: The aim of the Assessment Task 1 is to investigate the aftereffect of 1)temperature, 2)pH and 3)substrate absorption on the action of agitator such as catalase on hydrogen peroxide. Background knowledge: Enzymes are amoebic catalysts composed of proteins that abetment bacilli in facilitating metabolic reactions after ability any change themselves. Enzymes are acute to their ambiance and so charge abide aural a abiding ambit of factors ( pH, temperature , substrate absorption etc) for them to function. Any deviations from this abiding accompaniment can aftereffect in decreased ability or alike the denaturing (destruction) of the enzyme. What affects enzymes: 1)Temperature- Enzymes stop alive if the temperature rises aloft 40? C. Accretion the temperature alters the 3D appearance and so the agitator can no best fit the substrate. 2)pH- They assignment best in aloof altitude neither acerb nor alkaline. 3)Substrate absorption – Accretion the substrate concentration, increases the activiy of the enzymes till it alcove an optimal point aloft which there is no change in the agitator acitivity. Catalase Enzyme: The action of an agitator can be approved application liver, which contains the enzyme, catalase. Hydrogen achromatize break bottomward boring to anatomy baptize and oxygen. One atom of Catalase can accord with six actor molecules of Hydrogen Achromatize in 1 minute. This breakdown happens rapidly in the present of the Catalase and Oxygen gas evolves rapidly and can be activated with a aglow splint or ascent bubbles (variable). Changes in the temperature, acidity (pH) and absorption of the hydrogen achromatize will affect the bulk of the reaction. The ascendancy was to accept a analysis tube of aloof substrate after any enzymes present. The authority would be to analysis anniversary capricious in abreast after bond any of the 3 variables namely, the pH, acting and substrate concentration. The bulk of catalase and hydrogen achromatize will abide the aforementioned in all the analysis tubes. Hypothesis: The antecedent is that back hydrogen achromatize break bottomward into baptize and oxygen gas because of the enzyme, it is accepted that with change in temperature of the catalase, oxygen bubbles would form. Apparatus / Equipment used: -test tubes & analysis tube racks -pipettes -Tweezers -Ruler -Water baths (for temperature control) -Ice brazier -Thermometer -Beakers -Hotplates -Measuring butt -Vinegar -Bi-Carb Soda -pH cardboard -pH meters Cardboard towels to awning up spills -Pen and cardboard to almanac after-effects Ingredients used: -Liver ( agitator alleged catalase) -Hydrogen Achromatize Equipment setup: The analysis tubes were bureaucracy up in a analysis tube rack. Ice brazier to air-conditioned and hot baptize brazier to balmy were additionally kept in readiness. Experiment 1 (Temperature): Procedure: 1)I put on the assignment shirt, goggles, gloves and cossack as a assurance measure. 2)I chopped up 3 according pieces of liver. 3)I placed 1 allotment of alarmist into one analysis tube each. 4)I able 3 analysis tubes anniversary absolute 10ml of hydrogen peroxide. 5)I bureaucracy a baptize baths with 100 Celcius temperature, for temperature ascendancy application the thermometer, to ensure the actual temperature was maintained. 6)I placed 2 analysis tubes absolute alarmist and hydrogen achromatize anniversary into the baptize bath. )When the actual temperature was reached, I bound transferred the alarmist application tweezers into the analysis tube absolute hydrogen achromatize from the aforementioned baptize ablution 8)I looked for any oxygen bubbles ascent up in the analysis tube and abstinent the acceleration application a adjudicator 9)I again the aloft accomplish with 350 Celcius temperature. 10)I again the aloft accomplish with 350 Celcius temperature. 11)I accustomed the analysis tube capacity to air-conditioned bottomward afore administration off the aqueous decay into the bore with affluence of baptize and the solid decay capacity anxiously into the adapted bin. 12)I rinsed all accoutrement acclimated and broiled them for approaching use. Results of Experiment 1: It was empiric that with 100 C, the temperature was too low and there was not abundant calefaction for Catalase to catalyse the acknowledgment well. At 350C temperature, the bubbles produced barm and it appeared like all the enzymes were catalyzing reactios. Back the temperature rose to 500C, the bubbles went down, advertence that the temperature was too high, consistent in a breakdown of the agitator alleged denaturation. The after-effects back advised resulted in a alarm shaped curve. As temperature increases so to does the alive activity of the agitator and substrate molecules which about collide. The abundance of collisions increases as the temperature increases appropriately initially accretion the bulk of reaction. This occurs up to a best bulk of acknowledgment and the temperature at which the best bulk of acknowledgment is accomplished is referred to as the optimum temperature. Aloft the optimum temperature, accretion temperature increases the alive activity of the molecules to the point that the three-dimensional appearance of the agitator can be lost. Appropriately the appearance of its alive armpit changes and can no best bind to the substrate, abbreviation the bulk of acknowledgment aloft the optimum temperature.

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